Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUNB

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A
cell line
MCF10A
cell type
Normal human breast epithelial cell
chip antibody
JunB (CST, #3753)
treatment
TGF-beta (+,24h)
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6672407
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed using the SimpleChIP Enzymatic Chromatin IP kit (9003, Cell Signaling Technologies), according to the manufacturer's instructions. The antibodies used for ChIP were anti-H3K4me3 (9751), anti-H3K27c (8173), anti-Smad2 (5339), and anti-c-Jun (9165), which were purchased from Cell Signaling Technologies. ChIP-seq libraries were prepared using the KAPA HyperPrep Kit.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
43374743
Reads aligned (%)
88.4
Duplicates removed (%)
8.6
Number of peaks
5894 (qval < 1E-05)

hg19

Number of total reads
43374743
Reads aligned (%)
87.6
Duplicates removed (%)
9.1
Number of peaks
5245 (qval < 1E-05)

Base call quality data from DBCLS SRA